Rabbit anti-PLK1 Antibody, Affinity Purified

SKU: orb1530467

Description

PLK1 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:10,000; IP - 2 - 4 µg/mg lysate; ICC-IF - 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 325 and 375
• Target: PLK1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLK; serine/threonine-protein kinase 13; STPK13; serine/threonine-protein kinase PLK1; cell cycle regulated protein kinase; PLK-1; Polo-like kinase 1; polo (Drosophia)-like kinase

Detection of human PLK1 by western blot.Samples: Whole cell lysate from HeLa and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-PLK1 antibody orb1530467 at 0.1 µg/ml.

Detection of human PLK1 by WB and IP.Samples: HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody orb1530467 used at the indicated concentrations for WB.

Detection of human PLK1 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-PLK1 used at a dilution of 1:1, 000. Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.

Quick Database Links

Gene Symbol

PLK1

UniProt

P53350

RefSeq (Protein):NP_005021.2