Rabbit anti-Rictor Antibody, Affinity Purified

SKU: orb1523196

Description

Rictor Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. orb1523196 has not performed satisfactorily when used for WB of Rictor in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Rictor.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1 and 50
• Target: Rictor
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hAVO3; TORC2-specific protein AVO3; rapamycin-insensitive companion of mTOR; PIA; AVO3 homolog; AVO3; pianissimo

Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Rictor antibody orb1523196 used for IP at 6 µg per reaction.

Detection of human Rictor by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. orb1523196) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Quick Database Links

Gene Symbol

Rictor

UniProt

Q6R327

RefSeq (Protein):NP_689969.2