Rabbit anti-SOX10 Antibody, Affinity Purified

SKU: orb1520418

Description

SOX10 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 416 and C-terminus
• Target: SOX10
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

transcription factor SOX-10; SRY-related HMG-box gene 10; WS4C; WS4; SRY (sex determining region Y)-box 10; PCWH; dominant megacolon, mouse, human homolog of; DOM; WS2E

Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from Malme-3M, HEK293T, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOX10 antibody orb1520418 used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse SOX10 by western blot. Samples: Whole cell lysate (15 µg) from TCMK-1, B16-F10, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOX10 antibody orb1520418 used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human SOX10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOX10 antibody orb1520418 used for IP at 1 µg per reaction.

Quick Database Links

Gene Symbol

SOX10

UniProt

P56693

RefSeq:P56693.1