Rabbit anti-WDR5 Antibody, Affinity Purified

SKU: orb1526637

Description

WDR5 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-WDR5 antibody A302-429A.; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 75 and 125
• Target: WDR5
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WD repeat-containing protein 5; BIG-3; BMP2-induced 3-kb gene protein; CFAP89; cilia and flagella associated protein 89; SWD3; SWD3, Set1c WD40 repeat protein, homolog

Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody orb1526637 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human and mouse WDR5 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody orb1526637 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. WDR5 was successfully immunoprecipitated by rabbit anti-WDR5 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human WDR5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-WDR5 (Cat. No. orb1526637) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

WDR5

UniProt

P61964

RefSeq (Protein):NP_060058.1