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Rabbit IgG Fab

SKU: orb2652745

Description

Rabbit IgG Fab

Research Area

,PSC Type: Protein

Images & Validation

Tested ApplicationsELISA, IHC, SDS-PAGE, WB
Application Notes
Rabbit IgG Fab Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments

Key Properties

SourceRabbit
Biological OriginRabbit
IsotypeIgG Fab
ConjugationUnconjugated
PurityRabbit IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(ab’)2. No reaction was observed against anti-Rabbit IgG F(c) or anti-Papain.

Storage & Handling

StorageStore vial at 4° C prior to opening. Stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration5.0 mg/mL
Expiration Date6 months from date of receipt.
Hazard InformationThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Biorbyt are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Biorbyt, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
DisclaimerFor research use only

Alternative Names

Rabbit Immunoglobulin Fab, F(ab), Fragment antigen-binding

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol