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Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347681

Description

RABBIT IgG (H&L) antibody (Alkaline Phosphatase)

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Native Protein

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, WB
Dilution RangeELISA: 1:3,000 - 1:25,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
ReactivityRabbit
Application Notes
Anti-Rabbit IgG (H&L) alkaline phosphatase conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
PurityAnti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.
ConjugationAP

Storage & Handling

StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.1% (w/v) Sodium Azide
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, goat anti-Rabbit IgG Alk Phos Conjugated Antibody, goat anti-rabbit IgG heavy and light chain Antibody conjugated to Alkaline phosphatase

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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DOT
Dot Blot
View Protocol

Available Sizes

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1 mg
$ 370.00
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