Randox - Matrix Metalloproteinase-9 (MMP-9)

SKU: RCP9311

Description

Matrix metalloproteinases are a family of zinc and calciumdependent endopeptidases, which degrade extracellular matrix proteins. MMP-9 is secreted as a 92kDa zymogen. Cleavage of pro-MMP-9 results in the active enzyme with a molecular weight of ~82kDa. MMP-9 has a gelatinbinding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodelling, wound healing, tumour growth and metastasis.

Research Area

,For R&D Purposes

Images & Validation

• Application Notes:
  ELISA, Western Blotting, etc.

Key Properties

• Source: E.coli -derived human recombinant protein
• Expression System: E.coli
• Tag: N terminal containing a HIS tag
• Protein Length: Asp113- Glu450
• MW: 42.03 kDa
• Purity: > 95 %, by SDS-PAGE under reducing conditions

Storage & Handling

• Storage: at -20 °C
• Form/Appearance: Liquid
• Buffer/Preservatives: 20 mM Tris HCl pH 8, 50% Glycerol
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Matrix metalloproteinase 9, gelatinase B, CLG4B, GELB

Quick Database Links

UniProt

P14780