Randox - Mitochondrial Glycerol-3-Phosphate Dehydrogenase (mGPD) (Western Blot Control)

SKU: RCP9300

Description

Mitochondrial glycerol-3-phosphate dehydrogenase (mGPD) is a Ca2+ -sensitive, FAD-binding protein, located on the outer surface of the inner mitochondrial membrane. mGPD catalyses the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Two isoforms have been described for mGPD. Isoform 1 comprises 727aa residues, whereas isoform 2 lacks 126aa residues of the N-terminus. Deficiency of mGPD appears to contribute to the impairment of glucose-stimulated insulin release in several animal models of non-insulin dependent diabetes mellitus.

Research Area

,For R&D Purposes

Images & Validation

• Application Notes:
  ELISA, Western Blotting, etc.

Key Properties

• Source: E.coli -derived human recombinant protein
• Expression System: E.coli
• Tag: N terminal containing a HIS tag
• Protein Length: Ala43-Tyr600
• MW: 66.26 kDa
• Purity: > 95 %, by SDS-PAGE under reducing conditions

Storage & Handling

• Storage: at -20 °C
• Form/Appearance: Liquid. Supplied in 1x Laemmli Buffer (25mM Tris-HCl pH 6.8, 50mM DTT, 1% (w/v) SDS, 0.1% (w/v) Bromophenol Blue, 2.5% Glycerol).
• Buffer/Preservatives: 1X Laemmli buffer
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glycerol-3-Phosphate Dehydrogenase 2, GPD2, Glycerophosphate Dehydrognase-2 Ca(2+ ) - Responsive Mitochondrial FAD-Linked, Mitochondrial GPD, GPDM, GPD-M, GPDH-M, mtGPD, GDH2.

Quick Database Links

UniProt

P43304