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RANKL rabbit pAb

SKU: orb767009

Description

Rabbit polyclonal antibody to RANKL

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

Tested ApplicationsELISA, IHC-P, WB
Dilution RangeWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human TNFSF11. AA range:268-317
Molecular Weight35
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-TNFSF11 antibody, anti-OPGL antibody, anti-RANKL antibody, anti-TRANCE antibody, anti-Tumor necrosis factor ligand superfamily member 11 antibody, anti-Osteoclast differentiation factor antibody, anti-ODF antibody, anti-Osteoprotegerin ligand antibody, anti-OPGLReceptor activator of nuclear factor kappa-B ligan antibody

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RANKL rabbit pAb

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

RANKL rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

RANKL rabbit pAb

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

RANKL rabbit pAb

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, RANKL Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

RANKL rabbit pAb (orb767009)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 350.00
DispatchUsually dispatched within 5-10 working days
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