Rat ATF6(Activating Transcription Factor 6) ELISA Kit

SKU: orb1146787

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 6(ATF6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Activating Transcription Factor 6(ATF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 6(ATF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Transcription Factor 6(ATF6) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

,Product Categories/ELISA Kits,Signal transduction;Developmental science;

Images & Validation

• Application Notes:
  Standard: 20 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATF6 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Range: 0.32-20 ng/mL
• Sensitivity: 0.126 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Quick Database Links

UniProt

G3V909