Rat GCSF protein

SKU: orb80033

Description

Granulocyte MCSF Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GMCSF Rat Recombinant is purified by proprietary chromatographic techniques.

Images & Validation

• Tested Applications: FA, HPLC, SDS-PAGE
• Application Notes:
  < strong>Experiment Notes: GMCSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated the PC GENE computer analysis program of protein sequences (telliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of GM-CSF as Reference Standard.

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Key Properties

• Biological Origin: E.coli
• Biological Activity: The ED50 range 0.001-0.01 ng/ml, determined by the dose dependent proliferation of murine MC/9 cells.
• Target: rGM CSF
• Conjugation: Unconjugated
• Purity: > 95.0% as determined by(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: GM-CSF Rat was lyophilized with no additives.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pluripoietin-alpha protein, Molgramostin protein, Sargramostim protein

Quick Database Links

Gene Symbol

rGM CSF