Rat Glycated Hemoglobin A1c (HbA1c) ELISA Kit

SKU: orb779228

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Hemoglobin A1c(HbA1c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c(HbA1c).

Research Area

Product Categories/ELISA Kits,Diagnosis of diabetes mellitus

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 1000 μg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Rat
• Sample Types: plasma and erythrocyte lysates
• Assay Time: 2h
• Range: 15.63-1000 μg/mL
• Sensitivity: 4.25 μg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Glycosylated Hemoglobin ELISA Kit, Hemoglobin A1c ELISA Kit, Hb1c ELISA Kit, HbAIc ELISA Kit, HbAIc ELISA Kit