RBM22 Antibody (Center)

SKU: orb33151

Description

Rabbit polyclonal antibody to RBM22. The protein is predominantly expressed in spleen, and is localized to the nucleus. RBM22 is involved in the first step of pre-mRNA splicing also in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Developmental Biology,Primary Antibodies;mRNA processing

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB35239
• Immunogen: Synthetic Peptide
• Target: RBM22
• Molecular Weight: 46896
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Pre-mRNA-splicing factor RBM22 antibody, anti-RBM22 antibody, anti-ZC3H16 antibody

RBM22 Antibody (Center) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the RBM22 antibody detected the RBM22 protein (arrow).

Western blot analysis of RBM22 (arrow) using rabbit polyclonal RBM22 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM22 gene.

Western blot analysis of lysates from zebra fish muscle, Zebrafish tissue lysate (from left to right), using RBM22 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

RBM22 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM22 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

RBM22

UniProt

Q9NW64

RefSeq (Protein):NP_060517.1