RCC1 monoclonal antibody (M02), clone 1C1

SKU: orb2294476

Description

Mouse monoclonal antibody raised against a partial recombinant RCC1.

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 1C1
• Immunogen: RCC1 (AAH07300, 312 a.a. ~ 421 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: EGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged RCC1 is 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to RCC1 on HeLa cell. [antibody concentration 15 ug/ml].

Immunoprecipitation of RCC1 transfected lysate using anti-RCC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RCC1 MaxPab rabbit polyclonal antibody.

Western Blot analysis of RCC1 expression in transfected 293T cell line by RCC1 monoclonal antibody (M02), clone 1C1. Lane 1: RCC1 transfected lysate(45 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (37.73 KDa).

Quick Database Links

RefSeq:AAH07300