Ready-To-Use RFP Antibody Pre-adsorbed

SKU: orb535100

Description

DsRed antibody

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Recombinant Protein

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 1:1,000
• Reactivity: Other
• Application Notes:
  Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and tested in ELISA and in western blot using 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient to run 10 western blots. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. Optimal titers for these applications should be determined by the researcher.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
• Purity: RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.005mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody

Quick Database Links

UniProt

Q9U6Y8