Recombinant AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody

SKU: orb607305

Description

Mouse monoclonal antibody to AKT1 (Prognostic Marker for Neuroendocrine Tumors)

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Neuroscience,Signaling Pathways,Cancer,Developmental Biology,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Signaling Pathways/Apoptotic,AKT Signaling|BBB VCAM-1 Signaling|Breast Cancer|Cardiovascular|Colon Cancer|Cytokine Signaling|Developmental Biology|Immunology|Infectious Disease|Lung Cancer|MAPK Signaling|Neuroinflammation|Nuclear Marker|Ovarian Cancer|Signal Transduction|Transcription Factors

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7 cells. PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rAKT1/2491
• Immunogen: Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)
• Target: AKT1
• Source/Expression System: HEK293 cells
• Molecular Weight: 62kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749

NCBI Gene ID

207