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Recombinant Bax (Apoptosis Marker) Antibody

SKU: orb2310258

Description

Recombinant Bax (Apoptosis Marker) Antibody

Research Area

,AKT Signaling|Apoptosis|Autophagy|Breast Cancer|Cardiovascular|Colon Cancer|Complement System|Lung Cancer|Ovarian Cancer|Signal Transduction|Transcription Factors

Images & Validation

Tested ApplicationsWB
Dilution RangeImmunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: Jurkat, K562, HL-60, or HeLa cells. Reed-Sternberg cells in Hodgkin s lymphoma.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.BAX/8960R
ImmunogenRecombinant fragment (around aa1-100) of human BAX protein (exact sequence is proprietary)
TargetBAX
Source/Expression SystemCHO cells
Molecular Weight21kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab WITHOUT BSA and Azide at 1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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