Recombinant Calnexin (Endoplasmic Reticulum Marker) Antibody

SKU: orb2317143

Description

Recombinant Calnexin (Endoplasmic Reticulum Marker) Antibody

Research Area

,Cytokine Signaling|Immunology|Infectious Disease

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: CANX/9147R
• Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
• Target: CANX
• Source/Expression System: CHO cells
• Molecular Weight: 90kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90

Quick Database Links

Gene Symbol

CANX

UniProt

P27824

NCBI Gene ID

821