Recombinant CD3e (T-Cell Marker) Antibody

SKU: orb2316247

Description

Recombinant CD3e (T-Cell Marker) Antibody

Research Area

,Cardiovascular|Hematopoietic Stem Cells|Immunology|PD-1 blockade immunotherapy

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Jurkat and MOLT-4 cells. Human tonsil or lymph node

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: C3e/3125R
• Immunogen: Recombinant human CD3e fragment (around aa 23-119) (exact sequence is proprietary)
• Target: CD3E
• Source/Expression System: HEK293 cells
• Molecular Weight: 20-25kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766

NCBI Gene ID

916