Recombinant CTNND1 Antibody / p120 Catenin / Catenin delta 1

SKU: orb2635414

Description

Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. p120 is a proliferation-associated nucleolar protein found in most human malignant tumors, but not in resting normal cells. In colorectal cancer the altered localization of p120 catenin corresponds with loss of cytoplasmic localization of E-cadherin. Studies have shown accurate categorization of ductal vs. lobular neoplasia in the breast was achieved with p120 staining. p120 expression further clarifies the separation of low-grade ductal carcinoma in situ from lobular neoplasia. Studies also have shown that altered expression of p120 catenin antibody predicts poor outcome in invasive breast cancer.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CTNND1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rCTNND1/6903
• Immunogen: A portion of amino acids 868-968 was used as the immunogen for the recombinant CTNND1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant CTNND1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human stomach tissue glandular cells with recombinant CTNND1 antibody (clone rCTNND1/6903) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CTNND1 antibody (clone rCTNND1/6903) as confirmation of integrity and purity.

Quick Database Links

UniProt

O60716