Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293)

SKU: orb1516501

Description

Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293)

Research Area

Product Categories/Products/Proteins,Regulatory T Cells,CD,cd3,Human Lineage Negative

Images & Validation

• Application Notes:
  Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Key Properties

• Expression System: HEK293 cell
• Target: T-cell surface glycoprotein CD3 epsilon chain
• Tag: C-hFc
• Endotoxins: < 1.0 EU/μg as determined by LAL
• MW: Due to glycosylation,the protein migrates to 44-46 & 46-48 kDa based on Tris-Bis PAGE result. kDa
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• Protein Sequence: 20-198/198

Storage & Handling

• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Concentration: > 0.5 mg/ml
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3; CD3D; CD3-DELTA; T3D; CD3G;Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293)

Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml /14.6 ng/ml /13.6 ng/ml determined by ELISA.

Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml determined by ELISA.

The purity of Cynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma was greater than 95% as determined by SEC-HPLC.

ynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Quick Database Links

Gene Symbol

T-cell surface glycoprotein CD3 epsilon chain