Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody

SKU: orb1410496

Description

Mouse monoclonal antibody to KRT20 (Colorectal Epithelial Marker)

Research Area

,Product Categories/Antibodies,Developmental Biology

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HT29 cells. Human normal colon or colon adenocarcinoma

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: rKRT20/6536
• Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
• Target: KRT20
• Source/Expression System: HEK293 cells
• Molecular Weight: 46kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Quick Database Links

Gene Symbol

KRT20

UniProt

P35900

NCBI Gene ID

54474