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Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody
SKU: orb751378
Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Basal Cell Marker|Developmental Biology
Images & Validation
−
| Tested Applications | IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | KRT5/6399R |
| Immunogen | Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary) |
| Target | KRT5 |
| Source/Expression System | CHO cells |
| Molecular Weight | 58kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | 200 μg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-58kDa Cytokeratin antibody, anti-DDD1 antibody, anti-Epidermolysis Bullosa Simplex 2 (EBS2) antibody, anti-Keratin 5 antibody, anti-Keratin antibody, anti-Type II Cytoskeletal 5 antibody, anti-Keratin-5 antibody, anti-Type-II Cytoskeletal 5 antibody, anti-Type-II keratin Kb5 antibody
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