Recombinant Cytokeratin 8/18 Antibody

SKU: orb2319810

Description

Recombinant Cytokeratin 8/18 Antibody

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rKRT8.18/1346
• Immunogen: Recombinant full-length human KRT8 protein & Recombinant full-length human KRT18 protein
• Target: KRT8
• Source/Expression System: HEK293 cells
• Molecular Weight: 52.5kDa (CK8); 45kDa (CK18)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Quick Database Links

Gene Symbol

KRT8

UniProt

P05787

NCBI Gene ID

3856, 3875