Recombinant EpCAM / CD326 (Epithelial Marker) Antibody

SKU: orb2320635

Description

Research Area

,Stem Cell Differentiation

Images & Validation

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Tested Applications	FC, IF, IHC, WB
Dilution Range	Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: MCF-7 or HT29 cells. Human breast tumor

Key Properties

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Antibody Type	Recombinant Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	rMOC-31
Immunogen	Recombinant full-length human EpCAM protein
Target	EPCAM
Source/Expression System	CHO cells
Molecular Weight	40-43kDa
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Concentration	Purified Ab with BSA and Azide at 200ug/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Quality Guarantee

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Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

NCBI Gene ID

4072

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence