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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Endotoxins | Less than 0.1 EU/ug of rEqIL-2 Cys141Ser as determined by LAL method. |
| MW | Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 6.5. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Quick Database Links
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Protocol Information
Protein Handling and Storage Guide
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