Recombinant FOXA1 / HNF3A Antibody

SKU: orb2316885

Description

Recombinant FOXA1 / HNF3A Antibody

Research Area

,Cancer|Developmental Biology|Nuclear Marker|Signal Transduction|Stem Cell Differentiation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (0.5-1ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Reactivity: Human, Rat
• Application Notes:
  Positive Control: HeLa, HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: FOXA1/2230R
• Immunogen: Recombinant human FOXA1 protein fragment (around aa372-472) (exact sequence is proprietary)
• Target: FOXA1
• Source/Expression System: HEK293 cells
• Molecular Weight: 49kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A)

Quick Database Links

Gene Symbol

FOXA1

UniProt

P55317

NCBI Gene ID

3169