Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

SKU: orb2309294

Description

Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Research Area

,Cardiovascular|Endothelial Cell Marker|Neural Stem Cells|Neuroinflammation|Neuroscience|Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Formalin-fixed) (0.25-2ug/ml for 30 minutes at RT);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: T98G cells. Human brain or astrocytoma

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: rGFAP/9150
• Immunogen: Recombinant fragment (around aa100-300) of human GFAP protein (exact sequence is proprietary)
• Target: GFAP
• Source/Expression System: CHO cells
• Molecular Weight: ~50kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Quick Database Links

Gene Symbol

GFAP

UniProt

P14136

NCBI Gene ID

2670