Recombinant HLA-Pan (MHC II) Antibody

SKU: orb2314307

Description

Recombinant HLA-Pan (MHC II) Antibody

Research Area

,Immunology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Ramos or Raji cells. Human tonsil or lymph node

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: HLA-Pan/2967R
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Target: HLA-DPB1
• Source/Expression System: HEK293 cells
• Molecular Weight: 33-35kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5

Quick Database Links

Gene Symbol

HLA-DPB1

UniProt

P01920, P01908, P01909, P04440, P01903

NCBI Gene ID

3122, 3117, 3115