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Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody

SKU: orb751375

Description

Rabbit monoclonal antibody to HSPD1

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Infectious Diseases,Neuroscience,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Cardiovascular|Mitochondria Marker|Ovarian Cancer|Transcription Factors

Images & Validation

Tested ApplicationsIHC, WB
Dilution RangeWestern Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityBovine, Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Application Notes
Positive Control: HeLa or Hep2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.HSPD1/6498R
ImmunogenRecombinant human full-length HSP60 protein
TargetHSPD1
Source/Expression SystemCHO cells
Molecular Weight60kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin at 1.0mg/ml.
Concentration200 μg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-60kDa heat shock protein mitochondrial antibody, anti-Chaperonin antibody, anti-60-KD (CPN60) antibody, anti-GROEL antibody, anti-HLD4 antibody, anti-HSP65 antibody, anti-HuCHA60 antibody, anti-SPG13 antibody, anti-Mitochondrial matrix protein P1 antibody, anti-P60 lymphocyte protein antibody, anti-Short heat shock protein 60 Hsp60s1 antibody, anti-Spastic paraplegia 13 antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol

Recombinant HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody (orb751375)

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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