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Recombinant Human Cysteine-rich Angiogenic Inducer 61
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rHuCYR61 as determined by LAL method. |
| MW | Approximately 39.4 kDa, a single non-glycosylated polypeptide chain containing 357 amino acids. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CYR61 protein [orb358744]
Greater than 90% as determined by SDS-PAGE.
55.4 kDa
E.coli
20 μg, 100 μg, 1 mgHuman CYR61 protein [orb391607]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman CYR61 Protein [orb425008]
Greater than 95.0% as determined by SDS-PAGE.
Escherichia Coli
20 μg, 1 mg, 5 μgCYR61/CCN1 Protein, Human, Recombinant (hFc) [orb1961640]
98.00%
71 KDa (reducing condition)
1 mg, 500 μg, 50 μg, 10 μg

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Protocol Information
Recombinant Human Cysteine-rich Angiogenic Inducer 61 (orb1906264)
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