Recombinant Human EGF

SKU: orb1921839

Description

The epidermal growth factor (EGF) is a single-chain protein containing 53 amino acids; six of them are cysteine, forming disulfide linkages. Binding of EGF to EGF receptor (EGFR) is mediated through the N-terminal in the extracellular domain that is divided into four clefts. The EGF family of ligands includes eleven structurally related proteins, namely EGF, transforming growth factor α (TGF-α), amphiregulin (AREG), epigen (EPGN), heparin-binding EGF-like growth factor (HB-EGF), epiregulin (EREG), betacellulin (BTC), and the neuregulins (NRG1-4). These molecules have in common similar EGF-like motifs, and, due to their membrane-anchored nature, can act in a juxtacrine manner between two neighboring cells, or, in an autocrine/paracrine mode via proteolytic cleavage of the external EGF-like domain, which results in its release in the extracellular compartment. The EGF-like protein drives cellular signal transduction through the ErbB subclass of the Receptor Tyrosine Kinase superfamily, which consists of four members EGFR (also known as ErbB1), ErbB2, ErbB3, and ErbB4. EGF has long been known for its role in promoting proliferation of intestinal epithelial cells. EGF is produced by epithelial niche cells at the base of crypts in vivo and is routinely added to the culture medium to support the growth of intestinal organoids ex vivo. The binding of EGF or EGF-like proteins induces oligomerization of EGFR (ErbB1) with ErbB2/c-neu, ErbB3, and ErbB4, and subsequent phosphorylation of the receptor, leading to the transduction of different pathways: PLC-γ1, PI-3 Kinase, GAP-MAP-raf kinase. These pathways are involved in several functions, such as cell proliferation, migration and survival.

Images & Validation

• Application Notes:
  Application Notes: For research use only. Minimum Sample Size: 1 mg/vial

Key Properties

• Source: CHO Cell
• Expression System: Eukaryotic cell
• Biological Origin: Human
• Endotoxins: ≤10 EU/mg
• MW: 6.2kDa
• Purity: ≥95%

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze - thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -84°C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized Powder
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rHuEGF; Pro-epidermal growth factor; Urogastrone

Quick Database Links

UniProt

P01133

NCBI Gene ID

1950