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Recombinant Human Glial Cell Line-derived Neurotrophic Factor
SKU: orb1906241
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Description
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 x 107 IU/mg. |
| Endotoxins | Less than 0.1 EU/ug of rHuGDNF as determined by LAL method. |
| MW | Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains. |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, with 0.05 % Tween-20. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ATF, ATF-1
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Protein Handling and Storage Guide
Protein Handling Guide
Recombinant Human Glial Cell Line-derived Neurotrophic Factor (orb1906241)
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