Recombinant Human IFN gamma Protein

SKU: orb527076

Description

Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulatedwith phytohemagglutinin, and by others as a productofantigen-stimulatedlymphocytes ortuberculin-sensitized mouseperitoneal lymphocytes challenged with PPD.The resulting supernatants were shown toinhibit growth of vesicular stomatitisvirus. IFNγis a cytokine that is critical for innate and adaptive immunityagainst viral, some bacterial and protozoal infections. IFNγ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication ly, and most importantly from its immunostimulatoryand immunomodulatoryeffects. IFNγ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Key Properties

• Source: E. coli Gln24-Gly161
• Reactivity: Human
• Purification: > 95%, by SDS-PAGE quantitative densitometry by Coomassie? Blue Staining.
• Endotoxins: Less than 1 EU/μg of IFNγ as determined by LAL method.
• MW: 16.1KD
• Protein Sequence: QDPYV KEAEN LKKYF NAGHS DVADN GTLFL GILKN WKEES DRKIM QSQIV SFYFK LFKNF KDDQS IQKSV ETIKE DMNVK FFNSN KKKRD DFEKL TNYSV TDLNV QRKAI HELIQ VMAEL SPAAK TGKRK RSQML FRG

Storage & Handling

• Storage: Lyophilized recombinant human Interferon gamma remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFNγremains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P01579