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Recombinant Human Macrophage Migration Inhibitory Factor
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA. |
| Endotoxins | Less than 1 EU/ug of rHuMIF as determined by LAL method. |
| MW | Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids. |
| Purity | ≥95 by SDS-PAGE |
| Protein Sequence | MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. with 5% trehalose, 0.02% Tween-80. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human MIF protein [orb605235]
Greater than 90% as determined by SDS-PAGE.
41.3 kDa
Mammalian cell
1 mg, 20 μg, 100 μgRecombinant Human Macrophage Migration Inhibitory Factor, His [orb1905955]
> 95 % by SDS-PAGE and HPLC analyses.
Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 117 amino acids, with 6 x His at the C-terminus.
Escherichia coli
500 μg, 10 μg, 100 μgRecombinant Human Macrophage Migration Inhibitory Factor, Avi [orb1905956]
> 97 % by SDS-PAGE and HPLC analyses.
Approximately 14.3 kDa, a single non-glycosylated polypeptide chain containing 130 amino acids, with Avi tag at the C-terminus.
Escherichia coli
500 μg, 10 μg, 100 μgBiotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi [orb1905957]
> 97 % by SDS-PAGE and HPLC analyses.
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids, with 6 x His and Avi tag at the C-terminus.
Escherichia coli
500 μg, 10 μg, 100 μgHuman MIF protein [orb604247]
Greater than 90% as determined by SDS-PAGE.
16.3 kDa
E.coli
20 μg, 100 μg, 1 mg

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