Recombinant Human Perforin-1(PRF1)

SKU: orb1646467

Description

Plays a key role in secretory granule-dependent cell death, and in defence against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

Key Properties

• Expression System: Baculovirus
• Immunogen: 22-555aa
• Tag: N-terminal MBP-tagged and C-terminal 6xHis-tagged
• MW: 103.2 kDa
• Purity: Greater than 85% as determined by SDS-PAGE
• Protein Sequence: Full Length of Mature Protein

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
• Buffer/Preservatives: Tris-based buffer, 50% glycerol
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only