Recombinant Human Vitronectin

SKU: orb1921827

Description

Vitronectin (VN) is an abundant adhesive glycoprotein in blood plasma and is found associated with different extracellular matrix sites, the vessel wall, and tumor cells, particularly upon tissue remodeling, injury/repair, or under disease conditions. Plasma VN is a structurally labile molecule that may be converted into a multimeric/multivalent form by interaction with various (hemostatic) factors or through surface binding. Mature VN is composed of several functionally unique binding domains for different interaction partners along the protein sequence that are mainly concentrated within the amino- and carboxy-terminal parts of the protein. Starting at the amino-terminus, these are the ‘‘somatomedin-B’’ (SMB) domain, the Arg-Gly-Asp (RGD) adhesion motif followed by an adjacent acidic epitope (due to sulfation and phosphorylation sites) that is part of the binding site for high molecular weight kininogen (HK), a long connecting segment encompassing a collagen binding domain, followed by two substructured hemopexin-like domains within the carboxy-terminal portion of the protein. These distinct binding domains endow VN acts as a potent matricellular factor, coordinating cell migration with pericellular proteolysis and growth factor signaling at sites of tissue remodeling or in tumors. The glycosylated form of human VN (459 amino acid residues) has a molecular mass of 75 to 78 kDa and circulates as single-chain (65 kDa) and/or disulfide-bridged twochain (10 to 12 kDa) forms, the latter being genetically determined by the amino acid residue at position 381 and derived from proteolytic cleavage in the carboxy-terminal region. A Met381Thr polymorphism increases the risk by 18-fold of hemangioblastoma in patients with von-Hippel-Lindau gene defect, possibly related to enhanced tumor angiogenesis.

Images & Validation

• Application Notes:
  Application Notes: For research use only. Minimum Sample Size: 1 mg/vial

Key Properties

• Source: CHO Cell
• Expression System: Eukaryotic cell
• Biological Origin: Human
• Endotoxins: ≤10 EU/mg
• MW: 52.10 kDa
• Purity: ≥90%

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze - thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -100°C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized Powder
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

S-protein, Serum-spreading factor, V75,VN

Quick Database Links

UniProt

P04004

NCBI Gene ID

7448