Recombinant Ki-67 (Proliferating Cell Marker) Antibody

SKU: orb751385

Description

Rabbit monoclonal antibody to MKI67

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Neuroscience|Nuclear Marker

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Any actively proliferating cells. Human skin, tonsil or lymph node.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MKI67/4945R
• Immunogen: Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)
• Target: MKI67
• Source/Expression System: HEK293 cells
• Molecular Weight: 345kDa and 395kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: 200 μg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-KI-67 antibody, anti-Ki67 antibody, anti-KI-67 Antigen (KIA) antibody, anti-MKI67 antibody, anti-Proliferation related Ki-67 antigen antibody

Quick Database Links

Gene Symbol

MKI67

UniProt

P46013

NCBI Gene ID

4288