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Recombinant Murine Sonic Hedgehog

SKU: orb1905933
ActiveBiologically Active

Description

Recombinant Murine Sonic Hedgehog

Images & Validation

Key Properties

SourceEscherichia coli
Expression SystemEscherichia coli
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml.
EndotoxinsLess than 1 EU/ugof rMuSHH as determined by LAL method.
MWApproximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Purity> 95 % by SDS-PAGE and HPLC analyses.
Protein SequenceIVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Storage & Handling

StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Form/AppearanceLyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SHH, HHG-1
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UniProt Details

No UniProt data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Available Sizes

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5 μg
$ 240.00
100 μg
$ 1,160.00
500 μg
$ 3,410.00
DispatchUsually dispatched within 5-10 working days
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