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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,Cardiovascular|Hematopoietic Stem Cells|Immunology
Images & Validation
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| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | MPO/33R |
| Immunogen | Recombinant fragment (around aa150-250) of human MPO protein (exact sequence is proprietary) |
| Target | MPO |
| Source/Expression System | HEK293 cells |
| Molecular Weight | heavy-light promoter: 72kDa; dimer: 140kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | 200 μg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-84 kDa / 89 kDa myeloperoxidase antibody, anti-EC 1.11.1.7 antibody, anti-EC1.11.2.2 antibody, anti-fj80f04 antibody, anti-MPO antibody, anti-mpx antibody, anti-myeloid-specific peroxidase antibody, anti-Myeloperoxidase antibody, anti-Myeloperoxidase heavy chain antibody, anti-Myeloperoxidase light chain antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry