Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody

SKU: orb2311644

Description

Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody

Research Area

,Cardiovascular|Developmental Biology|Stem Cell Differentiation

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Raji or U-937 cells. Human seminoma or testicular carcinoma

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: OCT4/8098R
• Immunogen: Recombinant fragment of human OCT-4/POU5F1 protein (exact sequence is proprietary)
• Target: POU5F1
• Source/Expression System: HEK293 cells
• Molecular Weight: 38.6kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OCT3; OCT3A; OCT4; OCT4A; OCT-3/4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Quick Database Links

Gene Symbol

POU5F1

UniProt

Q01860

NCBI Gene ID

5460