Recombinant Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Antibody

SKU: orb1410475

Description

Rabbit monoclonal antibody to Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

Research Area

,Product Categories/Antibodies,Apoptosis|Autophagy

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HDLM-2cells. Human spleen

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: PRF1/7077R
• Immunogen: Recombinant human Perforin-1protein fragment (around aa 413-552) (exact sequence is proprietary)
• Target: PRF1
• Source/Expression System: HEK293 cells
• Molecular Weight: 75kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF

Quick Database Links

Gene Symbol

PRF1

UniProt

P14222

NCBI Gene ID

5551