Recombinant Prostate Specific Antigen Antibody / PSA

SKU: orb2635060

Description

Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells.Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA.Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Prostate Specific Antigen antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: KLK3/7128R
• Immunogen: A portion of amino acids 150-250 was used as the immunogen for the recombinant Prostate Specific Antigen antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant Prostate Specific Antigen antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human prostate carcinoma tissue with recombinant Prostate Specfic Antigen antibody (clone KLK3/7128R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma tissue with recombinant Prostate Specific Antigen antibody (clone KLK3/7128R) at 2 ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P07288