Recombinant Topoisomerase I, Mitochondrial (TOP1MT) Antibody

SKU: orb1410322

Description

Mouse monoclonal antibody to Topoisomerase I, Mitochondrial (TOP1MT)

Research Area

,Product Categories/Antibodies,Cardiovascular|Mitochondria Marker

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rTOP1MT/488
• Immunogen: Recombinant full-length human TOP1MT protein
• Target: TOP1MT
• Source/Expression System: HEK293 cells
• Molecular Weight: 70kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase

Quick Database Links

Gene Symbol

TOP1MT

UniProt

Q969P6

NCBI Gene ID

116447