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Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody
SKU: orb2316285
Description
Research Area
,Nuclear Marker
Images & Validation
−
| Tested Applications | IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | rTOP2A/6629 |
| Immunogen | Recombinant human Topoisomerase II alpha fragment (aa1352-1493) |
| Target | TOP2A |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 170kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A
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