Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

SKU: orb750912

Description

Mouse monoclonal antibody to VIM

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Infectious Diseases,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Cytokine Signaling|Immunology|Neural Stem Cells|Ovarian Cancer|Signal Transduction|Stem Cell Differentiation

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Bovine, Canine, Equine, Feline, Gallus, Human, Porcine, Rat
• Application Notes:
  Positive Control: U-87, Raji, Jurkat or HeLa cells. Human lymph node or tonsil.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rVIM/6575
• Immunogen: Recombinant full-length human Vimentin protein
• Target: VIM
• Source/Expression System: HEK293 cells
• Molecular Weight: 57-60kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: 200 μg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-VIM antibody

Quick Database Links

Gene Symbol

VIM

UniProt

P08670

NCBI Gene ID

7431