Recombinant Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody

SKU: orb750914

Description

Mouse monoclonal antibody to WT1

Research Area

Product Categories/Products/Antibodies/Primary Antibodies,Cancer,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Cardiac Stem Cells|Developmental Biology|Stem Cell Differentiation

Images & Validation

• Tested Applications: IHC
• Dilution Range: ELISA (For coating, order antibody without BSA); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: K562 cells. Human Wilm s tumor, mesothelioma or fetal kidney.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rWT1/6908
• Immunogen: Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary)
• Target: WT1
• Source/Expression System: HEK293 cells
• Molecular Weight: 49kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: 200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: 200 μg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-WT1 antibody, anti-AWT1 antibody, anti-FWT1 antibody, anti-GUD antibody, anti-NPHS4 antibody, anti-WAGR antibody, anti-Wilms tumor 1 antibody

Quick Database Links

Gene Symbol

WT1

UniProt

P19544

NCBI Gene ID

7490