REG3G Antibody (Center)

SKU: orb35898

Description

Rabbit polyclonal antibody to REG3G first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics,Primary Antibodies;Immunology;Inflammatory response;Immune system process

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: IHC-P: 1:50-100, WB: 1:1000, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB27078
• Immunogen: Synthetic Peptide
• Target: REG3G
• Molecular Weight: 19330
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Regenerating islet-derived protein 3-gamma antibody, anti-REG3G antibody, anti-PAP1B antibody

REG3G Antibody (Center) western blot analysis in SH-SY5Y cell line lysates (35 ug/lane). This demonstrates the REG3G antibody detected the REG3G protein (arrow).

Western blot analysis of lysate from SH-SY5Y cell line, using REG3G Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Anti-REG3G Antibody (Center) at 1:400 dilution + human kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-REG3G Antibody (Center) at 1:1000 dilution + recombinant protein lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-REG3G Antibody (Center) at 1:1000 dilution. Lane 1: mouse small intestine tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 20kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-REG3G Antibody (Center) at 1:2000 dilution. Lane 1: human kidney lysates. Lane 2: human lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-REG3G Antibody (Center) at 1:100 dilution. Lane 1: human kidney lysates. Lane 2: human pancreas lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

REG3G

UniProt

Q6UW15

RefSeq (Protein):NP_940850.1, NP_001008388.1, NP_001256969.1