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RFFL Antibody

SKU: orb344634

Description

RFFL antibody

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Recombinant Protein

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:4,000 - 1:20,000, IHC: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
ReactivityHuman
Application Notes
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
PurityThis protein A purified antibody is directed against human RFFL protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration5.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

rabbit anti-RFFL antibody, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq17432433

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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500 μg
$ 800.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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