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Description
Research Area
Product Categories/Products/Antibodies,Immunogen Type: Recombinant Protein
Images & Validation
−| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000, IF: 1:100, WB: 1:1,000 - 1:3,000 |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Gallus |
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
| Purity | RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−chicken anti-RFP antibody biotin conjugation, biotin conjugated chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)



